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. 2022 Aug 29;37(1):2357–2369. doi: 10.1080/14756366.2022.2116015

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The stability and anti-tumour activity in vitro. (A) The absorption spectrum of B2 at different times was detected by ultraviolet spectra assay. (B) The change in concentrations of B2 and curcumin in H460 cells. (C) The cell survival rate was detected by MTT assay after 12, 24, 36, and 48 h treatment of B2 and curcumin. (D) Clonogenic assay of H460 cells treated with B2 and curcumin as indicated. (E) H460 cells were treated with increasing concentrations of B2 and curcumin. The effect of B2 and curcumin in cell cycle was detected using flow cytometer. (F) The cell apoptosis was detected following the treatment of B2 and curcumin. (G) Western blot was performed to detect the effect of B2 on bcl-2 protein after 20 h treatment. (H) The changes in cell morphology were detected after 28 h of B2 treatment. (I) Western blot was performed to detect the effect of B2 on the pyroptosis-related proteins.