TABLE III.

Mean structural parameters and 2DFS linewidths determined for the (Cy3)₂ dimer-labeled −1 “fork” ss–dsDNA construct. The parameters determined from model analyses of spectroscopic data are the same as defined in Table II.

Optimized parameters for (iCy3)2 dimer −1 “fork” ss–dsDNA constructs
From absorbance and CD optimization					From 2DFS optimization
T (°C)	$\overline{J}$ (cm−1)	${\overline{\varphi }}_{AB}$ (deg)	${\overline{\theta }}_{AB}$ (deg)	${\overline{R}}_{AB}$ (Å)	σI (cm−1)	ΓH (cm−1)
1	−340	96.1 ± 0.3	39.9 + 2.2/−2.4	5.0 ± 0.1	187.3 + 14.5/−15.3	118.35 + 12.2/−9.6
5	−339	97.5 + 0.2/−0.7	35.5 + 1.8/−7.6	5.3 + 0.2/−0.1	207.6 + 22.3/−19.6	105.1 + 15.1/−11.7
15	−537	101 ± 0.7	47.3 + 4.2/−4.5	5.3 ± 0.1	217.7 + 39.1/−37.8	87.3 + 17.1/−18.3
23	−489	101 ± 0.8	44.2 + 5.4/−6.5	5.4 ± 0.1	187.3 + 20.5/−22.0	113.9 + 13.6/−11.6
35	−390	101 ± 0.7	44.4 + 6.1/−7.4	6.1 ± 0.1	192.4 + 19.8/−18.1	113.9 + 13.8/−10.6
45	−352	96 ± 0.5	58.2 + 3.2/−3.5	5.6 ± 0.1	187.3 + 17.7/−15.0	113.9 + 11.8/−9.5