Cellular levels of FliG, as measured by immunoblotting using polyclonal anti-FliG antibody, in the fliG null strain MKM1 transformed with the plasmids shown. v (vector), pUC18; wt (wild type), pGMK1000; Δ1, etc., pGΔ1, etc. Molecular mass markers are indicated on the right in kilodaltons.