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. 2022 Sep 6;219(11):e20220551. doi: 10.1084/jem.20220551

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© 2022 Shemesh et al.

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Figure S5. — Influence of CD16 or NKG2C stimulation on adoptive FcRγ^−/low NK cells. (A i) NK cell proliferation (CTV assay) of sorted CD57⁺ vs. unsorted CD57^± adaptive vs. nonadaptive NK cells from HDs #1, #2 after stimulation with anti-CD16 + IL-2 (6 U/ml). (A ii) Normalized pS6 gMFI levels after 24 h of the indicate stimulation in the defined mature NK cell subsets. Mean ± SD. Paired t test, two tails, *, P < 0.05; **, P < 0.01. (B) FcRγ levels of NKG2A⁻ NK cells in donors with adoptive NK cells (Fig. S2). Cells were stimulated with anti-NKG2C-coated or mouse IgG1 isotype-matched control-coated beads for 6 d in the presence of increasing IL-2 concentrations. Mean ± SD. Paired t test, parametric. *, P < 0.05.