Fig. 2. Bladder cancer cell proliferation, motility and glycolysis were impeded after circ_0003221 knockdown. (A) Reverse transcription-quantitative polymerase chain reaction was performed for quantifying circ_0003221 in 5637 and T24 cells with si-NC or si-circ_0003221 transfection. EDU assay or colony formation assay (B, C) and transwell assay (D, E) were performed for examination of proliferation and migration/invasion (crystal violet, ×100 magnification). Glucose consumption (F) and lactate production (G) through kits were performed for glycolysis evaluation. (H, I) Western blot was performed for examining Cyclin D1, MMP2 and PKM2. DAPI, diamidine phenylindole. ^***p<0.001.