Fig. 5. Circ_0003221 mediated the DHCR24 expression by targeting miR-892b. (A) The site analysis of DHCR24 and miR-892b was conducted by Targetscan. (B) The expression level of DHCR24 in GEPIA dataset. DHCR24 level analysis was carried out via reverse transcription-quantitative polymerase chain reaction and western blot in bladder cancer tissues (C, D) and cells (E, F). (G, H) Dual-luciferase reporter assay was administrated for analyzing miR-892b and DHCR24 3’UTR combination in 5637 and T24 cells. (I) Western blot was applied for miR-892b quantification in anti-NC or anti-miR-892b transfected 5637 and T24 cells. (J, K) DHCR24 protein determination was conducted using western blot after si-circ_0003221 and anti-miR-892b co-transfection. DHCR24, 24-dehydrocholesterol reductase; UTR, untranslated region; WT, wild-type; MUT, mutant-type. ^**p<0.01, ^***p<0.001.