Fig. 6. DHCR24 overexpression relieved the function of si-circ_0003221 in bladder cancer cells. (A) DHCR24 protein measurement was carried out through western blot following transfection with Vector or DHCR24. EDU assay (B) or colony formation assay (C) and transwell assay (D, E) were applied to analyze the proliferation ability and cell motility after si-NC, si-circ_0003221, si-circ_0003221+Vector or si-circ_0003221+DHCR24 transfection in 5637 and T24 cells. Glycolysis was tested by glucose uptake (F), lactate production (G) and marker detection (H, I). DHCR24, 24-dehydrocholesterol reductase. ^**p<0.01, ^***p<0.001.