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. 2022 Aug 11;25(9):1149–1162. doi: 10.1038/s41593-022-01131-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a, Changes in cluster distribution after CRISPRi knockdown of targeted genes in iTF-Microglia. Distribution of cells according to the 37 targeted genes and non-targeting control (NTC) in clusters 1–9. b, Average differences of gene expression induced by CDK12 knockdown in cluster 9 compared to those in all other clusters. Genes encoding phosphatidylserine (PS) recognition receptors are labeled in magenta and Genes encoding MHC complex components are labeled in green.