Extended Data Fig. 6. Related to main Fig. 2.

(a-e) Global prediction of registration error in nanometers (nm) from Fig. 2c-g respectively. The CLEM precision error is 20 nm to 150 nm in the x.y axis, and importantly around 20 nm at the point of the alignment organelle (nucleus or mitochondria). Organelles such as autophagosomes, lysosomes and mitochondria are small in size (< 1.5 μm), whilst the nucleus is larger (<10μm). If the size of the organelle is larger relative to the precision error (at the point of alignment), the fluorescent signals are likely to originate within the identified subcellular region, as is the case for larger organelles, while the fluorescent signal may originate partially outside the EM imaging area for smaller organelles.