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. 2022 Sep 6;12:15110. doi: 10.1038/s41598-022-19063-1

Figure 2.

Figure 2

A clustered heatmap for rectal temperatures in broilers exposed to high ambient temperatures. Temperature panels included RTs at thermoneutral (NT) and heat stress conditions (HS). The heatmap was constructed using the package “ComplexHeatmap” of the R software version 4.0.3 (R Core Team, 2020). Each row represents an RT record and each column represents an experimental unit belonging to a specific treatment. Precisely, CTX refers to RT records at a day X of thermoneutral condition; similarly, HSX refers to RT records at a day X of HS challenge. The treatments are as follows: CON chicks hatched from control eggs without in ovo injection and incubated at standard temperature, CON + HS chicks hatched from control eggs without in ovo injection, incubated at standard temperature but exposed to HS, G10 + HS chicks hatched from eggs injected at embryonic day (ED) 17.5 with 0.6 mL of 10% GABA dissolved in distilled water and exposed to HS, TM + HS chicks hatched from thermally manipulated eggs exposed to 39.6 °C for 6 h daily from ED 10 to 18 and exposed to HS, G10 + TM + HS chicks hatched from eggs that received both previous treatments during incubation and exposed to HS.