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. 2022 Sep 6;11(1):51. doi: 10.1038/s41389-022-00430-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Gene ontology (GO) analysis for (A) biological processes and (B) cellular components of the downregulated differentially expressed genes (DEGs) identified through RNA-seq. C Heatmap of top 13 genes involved in the regulation of cell migration, decreased (blue) or increased (red) in expression level upon UHRF1 loss. D, F Quantification of relative distance migrated in scratch-wound assays for (D) osteosarcoma VC cells assayed with fresh media (FM) or conditioned media (24 h) from VC cells or UHRF1 KO cells, normalized to FM; E osteosarcoma UHRF1 KO cells assayed with FM or conditioned media from VC cells or UHRF1 KO cells, normalized to FM; F each of the osteosarcoma cell lines treated with DMSO, 10 µM GW4869 or 20 µM CPZ, normalized to DMSO control. Each data point is mean ± s.d. of ten measurements in triplicate samples. G qPCR analysis of PLAU mRNA levels in flank-implanted tumors from control (VC) and UHRF1 KO (KO) SJSA-1 cell lines. *P < 0.05 by paired two-tailed t test. H Quantification of relative distance migrated in scratch-wound assays for each of the osteosarcoma cell lines treated with DMSO, 150 µM amiloride, or 16.4 µM BC11 hydrobromide, normalized to DMSO control. Each data point is mean ± s.d. of ten measurements in triplicate samples. I Representative images from Transwell assays with cells stained with crystal violet comparing levels of invasion between cells treated with DMSO or 150 µM amiloride in SJSA-1. J Quantification of the number of cells invaded in Transwell invasion assay for each of the osteosarcoma cell lines treated with DMSO or 150 µM amiloride. Each data point is mean ± s.d. of triplicate samples. For all graphs: ns not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001 by unpaired two-tailed t test.