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. 2022 Sep 6;11(1):51. doi: 10.1038/s41389-022-00430-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Representative fluorescent images of in situ hybridizations using RNAscope on wild-type femurs or tumors from genetically engineered osteosarcoma mice using a probe against Uhrf1 (red). Nuclei were counterstained with DAPI (blue). Uhrf1 expression is detected in Tp53 cKO and Tp53/Rb1 DKO mouse tumors but is low or not detected in wt, Tp53/Uhrf1 DKO, and Tp53/Rb1/Uhrf1 TKO tumors. B Kaplan–Meier curves showing the survival of osteosarcoma mouse models. Mice bearing Rb1 mutations Tp53/Rb1 DKO: Osx-Cre; p53^lox/lox; Rb1^lox/lox (red; n = 53) have significantly shorter lifespan compared to Tp53 cKO: Osx-Cre; p53^lox/lox; Rb1^lox/lox (black; n = 39). This survival time was significantly increased in Tp53/Rb1/Uhrf1 TKO: Osx-Cre; p53^lox/lox; Rb1^lox/lox; Uhrf1^lox/lox (blue; n = 37) mice. ^†Three surviving mice were removed from the study to confirm tumor absence. Tp53/Uhrf1 DKO: Osx-Cre; p53^lox/lox; Uhrf1^lox/lox (green; n = 39) showed an overall survival comparable to Tp53 cKO (black; n = 39). Mantel–Cox test were used for curve comparisons. ns not significant, *P < 0.05, ****P < 0.0001. C Summary table for each osteosarcoma mouse model. ^††Age of mice (weeks) at earliest tumor detection via microCT and PET scans comparison: P < 0.05 by unpaired two-tailed t test; Tp53/Rb1 DKO (n = 4) and Tp53/Rb1/Uhrf1 TKO (n = 3).