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. 2000 Jun;182(11):3045–3054. doi: 10.1128/jb.182.11.3045-3054.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 2 — Double-reciprocal plots of binding data for chitinase A1 (a), A1ΔChBD (b), and ChBD_ChiA1 (c). The binding assay mixture contained 1 mg (dry weight) of regenerated chitin and from 1 to 70 μg of each protein (14 to 1,000 pmol of chitinase A1, 15 to 1,050 pmol of A1ΔChBD, and 154 to 10,770 pmol of ChBD_ChiA1) in 1 ml of 20 mM sodium phosphate buffer (pH 6.0). The binding assay was performed by keeping the binding assay mixtures on ice for 1 h for chitinase A1 and A1ΔChBD and 3 h for ChBD_ChiA1. [B], bound protein concentration; [F], free protein concentration.

FIG. 2 — Double-reciprocal plots of binding data for chitinase A1 (a), A1ΔChBD (b), and ChBD_ChiA1 (c). The binding assay mixture contained 1 mg (dry weight) of regenerated chitin and from 1 to 70 μg of each protein (14 to 1,000 pmol of chitinase A1, 15 to 1,050 pmol of A1ΔChBD, and 154 to 10,770 pmol of ChBD_ChiA1) in 1 ml of 20 mM sodium phosphate buffer (pH 6.0). The binding assay was performed by keeping the binding assay mixtures on ice for 1 h for chitinase A1 and A1ΔChBD and 3 h for ChBD_ChiA1. [B], bound protein concentration; [F], free protein concentration.