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Time courses of binding of ChBDChiA1 at pH 9.0 and in the presence of NaCl. Assay mixtures contained 6 mg (dry weight) of regenerated chitin and 2.3 nmol (15 μg) of ChBDChiA1 in 1 ml of 20 mM sodium phosphate buffer (pH 6.0) (□), 20 mM sodium phosphate buffer (pH 6.0) containing 0.5 M NaCl (■), or 20 mM Tris-HCl buffer (pH 9.0) (+).
