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Binding specificities of chitinase A1, A1ΔChBD, and ChBDChiA1. Binding assay mixtures contained 1 mg (dry weight) of various insoluble polysaccharides and 25 μg of protein in 1 ml of 20 mM Tris-HCl (pH 9.0). Assay mixtures were kept on ice for 24 h for binding.
