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. 2022 Aug 24;13:966455. doi: 10.3389/fendo.2022.966455

Table 1.

Primers for real-time PCR.

Name Accession No. Sequence Product length (bp)
GAPDH F: 5’- AGCCACATCGCTCAGACA -3’ 66
NM_002046.7 R: 5’- GCCCAATACGACCAAATCC -3’
TLR3 F: 5’- GCGCTAAAAAGTGAAGAACTGGAT -3’ 145
NM_003265.3 R: 5’- GCTGGACATTGTTCAGAAAGAGG -3’
TLR4 F: 5’- CCCTGAGGCATTTAGGCAGCTA -3’ 126
NM_138554.5 R: 5’- AGGTAGAGAGGTGGCTTAGGCT -3’
MYD88 F: 5’- GAGGCTGAGAAGCCTTTACAGG -3’ 129
NM_001172567.2 R: 5’- GCAGATGAAGGCATCGAAACGC -3’
IRAK1 F: 5’- TCAGAACGGCTTCTACTGCCTG -3’ 128
NM_001569.4 R: 5’- TACCCAGAAGGATGTCCAGTCG -3’
IRAK2 F: 5’- TCGAGTACCTGCATGGTCTGGA -3’ 114
NM_001570.4 R: 5’- CAGGACACAGATGAGCCATTGG -3’
IRAK4 F: 5’- ATGCCACCTGACTCCTCAAGTC -3’ 131
NM_001114182.3 R: 5’- CCACCAACAGAAATGGGTCGTTC -3’
IRF1 F: 5’- GAGGAGGTGAAAGACCAGAGCA -3’ 121
NM_002198.3 R: 5’- TAGCATCTCGGCTGGACTTCGA -3’
IRF2 F: 5’- TAGAGGTGACCACTGAGAGCGA -3’ 125
NM_002199.4 R: 5’- CTCTTCATCGCTGGGCACACTA -3’
IRF3 F: 5’- TCTGCCCTCAACCGCAAAGAAG -3’ 151
NM_001571.6 R: 5’- TACTGCCTCCACCATTGGTGTC -3’
IRF7 F: 5’- CCACGCTATACCATCTACCTGG -3’ 153
NM_001572.5 R: 5’- GCTGCTATCCAGGGAAGACACA -3’
IRF9 F: 5’- CCACCGAAGTTCCAGGTAACAC -3’ 123
NM_001385400.1 R: 5’- AGTCTGCTCCAGCAAGTATCGG -3’
IL1B F: 5’- TGATGGCTTATTACAGTGGCAATG -3’ 140
NM_000576.3 R: 5’- GTAGTGGTGGTCGGAGATTCG -3’
IL6 F: 5’- CAGGAGCCCAGCTATGAACT -3’ 85
NM_000600.5 R: 5’- AGCAGGCAACACCAGGAG -3’
CXCL8 F: 5’- AAGCATACTCCAAACCTTTCCA -3’ 123
(NM_000584.4) R: 5’- CCAGACAGAGCTCTCTTCCA -3’
CXCL10 F: 5’- GGTGAGAAGAGATGTCTGAATCC -3’ 134
NM_001565.4 R: 5’- GTCCATCCTTGGAAGCACTGCA -3’
CXCL11 F: 5’- AAGGACAACGATGCCTAAATCCC -3’ 116
NM_005409.5 R: 5’- CAGATGCCCTTTTCCAGGACTTC -3’
IL12A F: 5’- TGCCTTCACCACTCCCAAAACC -3’ 100
NM_000882.4 R: 5’- CAATCTCTTCAGAAGTGCAAGGG -3’
IFNB1 F: 5’- CTTGGATTCCTACAAAGAAGCAGC -3’ 146
NM_002176.4 R: 5’- TCCTCCTTCTGGAACTGCTGCA -3’
STAT1 F: 5’- CCATCCTTTGGTACAACATGC -3’ 71
NM_007315.4 R: 5’- TGCACATGGTGGAGTCAGG -3’
STAT2 F: 5’- CAGGTCACAGAGTTGCTACAGC -3’ 118
NM_005419.4 R: 5’- CGGTGAACTTGCTGCCAGTCTT -3’
STAT3 F: 5’- CTTTGAGACCGAGGTGTATCACC -3’ 133
NM_139276.3 R: 5’- GGTCAGCATGTTGTACCACAGG -3’
STAT5A F: 5’- GTTCAGTGTTGGCAGCAATGAGC -3’ 108
NM_012448.4 R: 5’- AGCACAGTAGCCGTGGCATTGT -3’
ISG15 F: 5’- CTCTGAGCATCCTGGTGAGGAA -3’ 136
NM_005101.4 R: 5’- AAGGTCAGCCAGAACAGGTCGT -3’
ISG20 F: 5’- ACACGTCCACTGACAGGCTGTT -3’ 137
NM_002201.6 R: 5’- ATCTTCCACCGAGCTGTGTCCA -3’
MX1 F: 5’- GGCTGTTTACCAGACTCCGACA -3’ 143
NM_001144925.2 R: 5’- CACAAAGCCTGGCAGCTCTCTA -3’
MX2 F: 5’- AAAAGCAGCCCTGTGAGGCATG -3’ 163
NM_002463.2 R: 5’- GTGATCTCCAGGCTGATGAGCT -3’
APOBEC3G F: 5’- ATGACACCTGGGTCCTGCTGAA -3’ 114
NM_021822.4 R: 5’- GAATCACGTCCAGGAAGCACAG -3’

F, Forward, R, Reverse.