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. 2000 Jun;182(11):3063–3071. doi: 10.1128/jb.182.11.3063-3071.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 1 — Sequential disruption of PMT6 alleles. (A) Schematic representation of the construction of the different alleles. The wild-type PMT6 gene and the PMT6 alleles disrupted by the hisG-URA3-hisG cassette or by hisG are shown. H, HindIII; N, NcoI; X, XhoI, S, SalI; P, PstI; B, BglII; A, Asp718. The fragment marked by asterisks was used as a probe for Southern analysis. (B and C) Southern blots of HindIII-BglII-digested chromosomal DNA of the following strains: SC5314 (PMT6/PMT6; lane 1 [B and C]); CAP2-2 (PMT6/pmt6Δ::hisG-URA3-hisG; lane 2 [B]); CAP2-23 (PMT6/pmt6Δ::hisG; lane 3 [B]); CAP2-239 (pmt6Δ::hisG-URA3-hisG/pmt6Δ::hisG; lane 4 [B]); CAP2-2391 (pmt6Δ::hisG/pmt6Δ::hisG; lane 5 [B]); CPP1 (PMT6/pmt6Δ::hisG-URA3-hisG; lane 2 [C]); CPP11 (PMT6/pmt6Δ::hisG; lane 3 [C]); CPP117 (pmt6Δ::hisG-URA3-hisG/pmt6Δ::hisG; lane 4 [C]); CPP1171 (pmt6Δ::hisG/pmt6Δ::hisG; lane 5 [C]).