TABLE 1.
C. albicans strain(s) or plasmid(s) | Genotype or description | Reference or source |
---|---|---|
Strains | ||
SC5314 | Prototrophic | 11 |
CAI4 | ura3Δ::imm34/ura3Δ::imm34 | 11 |
CAP1-3 | As CAI4, but PMT1/pmt1Δ::hisG-URA3-hisG | 41 |
CAP1-312 | As CAI4, but pmt1Δ::hisG-URA3-hisG/pmt1Δ::hisG | 41 |
CAP1-3121 | As CAI4, but pmt1Δ::hisG/pmt1Δ::hisG | 41 |
SS4 | CAI8 derivative; efg1::ADE2/(URA3-PCK1p)::EFG1 | 36 |
HLC67 | efg1/efg1 derivative of CAI4 | 23 |
CAP2-1, CAP2-2, CAP2-3, CAP2-4 | As CAI4, but PMT6/pmt6Δ::hisG-URA3-hisG | This work |
CAP2-23 | As CAI4, but PMT6/pmt6Δ::hisG | This work |
CAP2-234, CAP2-239 | As CAI4, but pmt6Δ::hisG/pmt6Δ::hisG-URA3-hisG | This work |
CAP2-2341, CAP2-2391 | As CAI4, but pmt6Δ::hisG/pmt6Δ::hisG | This work |
CPP1 | As CAP1-3121, but PMT6/pmt6Δ::hisG-URA3-hisG | This work |
CPP11 | As CAP1-3121, but PMT6/pmt6Δ::hisG | This work |
CPP112, CPP117 | As CAP1-3121, but pmt6Δ::hisG/pmt6Δ::hisG-URA3-hisG | This work |
CPP1123, CPP1171 | As CAP1-3121, but pmt6Δ::hisG/pmt6Δ::hisG | This work |
Plasmids (transformation vectors) | ||
pRC18 | URA3-marked CaARS vector | 36 |
pRC2312 | URA3-marked CaARS vector | 4 |
pLJ19 (pCPH1) | CPH1 under control of the ADH1 promoter | 7 |
pCaTPK2 (pTPK2) | URA3-marked CaARS vectors containing TPK2 | 35 |
pCCa4 (pCEK1) | URA3-marked CaARS vectors containing CEK1 | 7 |
pBI-HAHYD (pEFG1) | EFG1 fused with the HAa tag under the control of the PCK1 promoter | 35 |
p99 | pUC18 containing PMT6 | S. Scherer |
pCT30 | URA3-marked CaARS vector containing PMT1 | 41 |
pCT34, pCT35 | URA3-marked CaARS vectors containing PMT6 | This work |
pCT17 | As p99, but without the part of the MCSb between SacI and SphI | This work |
pCT25 | Plasmid carrying pmt6Δ::hisG-URA3-hisG disruption fragment | This work |
HA, hemagglutinin.
MCS, multiple cloning site.