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. 2000 Jun;182(11):3063–3071. doi: 10.1128/jb.182.11.3063-3071.2000

TABLE 1.

Strains and plasmids

C. albicans strain(s) or plasmid(s) Genotype or description Reference or source
Strains
 SC5314 Prototrophic 11
 CAI4 ura3Δ::imm34/ura3Δ::imm34 11
 CAP1-3 As CAI4, but PMT1/pmt1Δ::hisG-URA3-hisG 41
 CAP1-312 As CAI4, but pmt1Δ::hisG-URA3-hisG/pmt1Δ::hisG 41
 CAP1-3121 As CAI4, but pmt1Δ::hisG/pmt1Δ::hisG 41
 SS4 CAI8 derivative; efg1::ADE2/(URA3-PCK1p)::EFG1 36
 HLC67 efg1/efg1 derivative of CAI4 23
 CAP2-1, CAP2-2, CAP2-3, CAP2-4 As CAI4, but PMT6/pmt6Δ::hisG-URA3-hisG This work
 CAP2-23 As CAI4, but PMT6/pmt6Δ::hisG This work
 CAP2-234, CAP2-239 As CAI4, but pmt6Δ::hisG/pmt6Δ::hisG-URA3-hisG This work
 CAP2-2341, CAP2-2391 As CAI4, but pmt6Δ::hisG/pmt6Δ::hisG This work
 CPP1 As CAP1-3121, but PMT6/pmt6Δ::hisG-URA3-hisG This work
 CPP11 As CAP1-3121, but PMT6/pmt6Δ::hisG This work
 CPP112, CPP117 As CAP1-3121, but pmt6Δ::hisG/pmt6Δ::hisG-URA3-hisG This work
 CPP1123, CPP1171 As CAP1-3121, but pmt6Δ::hisG/pmt6Δ::hisG This work
Plasmids (transformation vectors)
 pRC18 URA3-marked CaARS vector 36
 pRC2312 URA3-marked CaARS vector 4
 pLJ19 (pCPH1) CPH1 under control of the ADH1 promoter 7
 pCaTPK2 (pTPK2) URA3-marked CaARS vectors containing TPK2 35
 pCCa4 (pCEK1) URA3-marked CaARS vectors containing CEK1 7
 pBI-HAHYD (pEFG1) EFG1 fused with the HAa tag under the control of the PCK1 promoter 35
 p99 pUC18 containing PMT6 S. Scherer
 pCT30 URA3-marked CaARS vector containing PMT1 41
 pCT34, pCT35 URA3-marked CaARS vectors containing PMT6 This work
 pCT17 As p99, but without the part of the MCSb between SacI and SphI This work
 pCT25 Plasmid carrying pmt6Δ::hisG-URA3-hisG disruption fragment This work
a

HA, hemagglutinin. 

b

MCS, multiple cloning site.