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. 2022 Aug 19;37(4):599–607. doi: 10.3803/EnM.2022.1549

Fig. 2.

Fig. 2.

A schematic diagram of free cortisol analysis in serum. Zinc sulfate is used to precipitate large proteins in serum and an aqueous buffer exchange is then performed to further precipitate the remaining small proteins and zinc ions in sample solution. Samples are vortexed and centrifuged before analysis using mass spectrometry or immunoassay, and only the supernatant is used.