TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant phenotype or genotype | Source or reference |
|---|---|---|
| Strains | ||
| R. sphaeroides | ||
| 2.4.1 | Wild type | 23 |
| FNRL | 2.4.1 derivative, ΔfnrL::ΩKmr; formerly JZ1678 | 27 |
| CCOP1 | 2.4.1 derivative, ccoP::ΩTpr | 18 |
| CCOP1FNRL | FNRL derivative, ΔfnrL::ΩKmrccoP::ΩTpr | This study |
| PRRBCA2 | 2.4.1 derivative, ΔprrCBA::ΩTpr | This study |
| E. coli | ||
| DH5α | (φ80dlacZΔM15) ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 | 8 |
| S17-1 | Pro− Res− Mod+recA; integrated plasmid RP4-Tc::Mu-Km::Tn7 | 21 |
| Plasmids | ||
| pCF1010 | Spr Str Tcr; lacZYA′ IncQ | 10 |
| pCFL | pCF1010 in which ∼1.5-kb HindIII fragment containing Tc resistance gene is deleted; Spr Str | M. Gomelsky |
| pSUP203 | pBR325 derivative; Mob+ Apr Cmr Tcr | 21 |
| pUI1637 | pUI1188 containing the ΩKm | 6 |
| pUI1680 | pUI1637 with a 1,017-bp BglII-NruI deletion replaced with a ∼1.7-kb EcoRI-DraI fragment from pSUP5TpMCS containing a gene encoding Tpr | This study |
| pJE2077 | pSUP203 derivative containing a 5,232-bp fragment with the prr region, with a 2,069-bp Tth111I-BspEI deletion replaced with an ΩTp | This study |
| pJE3153 | pBSII SK+::0.75-kb SmaI fragment with the hemZ control region from pUI1970 (26) | This study |
| pUI2801 | ccoP::ΩTp in pSUP203 | 18 |
| pCF200Km | Spr Str Kmr; IncQ, puc::lacZYA′ | 9 |
| pUI1063 | Spr Str Tcr; IncQ, hemA::lacZYA′ | 27 |
| pJE3170 | Spr Str Tcr; IncQ, hemZ::lacZYA′ | This study |
| pUI2762 | Spr Str; IncQ, hemN::lacZYA′ | 24 |
| pBCHE | Spr Str; IncQ, bchE::lacZYA′ | This study |