TABLE 3.
Promoter activities of reporter genes in R. sphaeroides strains grown under aerobic conditionsa
| Strain | β-Galactosidase activity (nmol/min/mg of protein)
|
||
|---|---|---|---|
| puc::lacZ | hemA::lacZ | bchE::lacZ | |
| 2.4.1 | 49.4 ± 1.9 | 6.1 ± 0.5 | 4.3 ± 1.7 |
| CCOP1 | 136.3 ± 4.2 | 44.0 ± 3.3 | 73.8 ± 8.1 |
| CCOP1FNRL | 186.6 ± 1.0 | 99.8 ± 1.4 | 68.8 ± 5.0 |
a
Strains were grown aerobically by sparging with 30% O2–69% N2–1% CO2 to an OD600 of 0.4 to 0.5. Plasmids pCF200Km, pUI1063, and pBCHE, carrying transcriptional fusions puc::lacZ, hemA::lacZ, and bchE::lacZ, respectively, were used to determine the promoter activities of the corresponding gene. All values are the averages of two independent determinations.