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. 2000 Jun;182(11):3081–3087. doi: 10.1128/jb.182.11.3081-3087.2000

TABLE 4.

Promoter activities of hemZ and hemN in R. sphaeroides FNRL and CCOP1FNRL mutants grown under aerobic conditionsa

Strain β-Galactosidase activity (nmol/min/mg of protein)
hemZ::lacZ hemN::lacZ
FNRL 5.2 ± 2.0 52.1 ± 1.8
CCOP1FNRL 5.2 ± 1.0 195.2 ± 1.4
a

Strains were grown aerobically by sparging with 30% O2–69% N2–1% CO2 to an OD600 of 0.4 to 0.5. Plasmids pJE3170 and pUI2762 carrying transcriptional fusions hemZ::lacZ and hemN::lacZ, respectively, were used to determine promoter activities of the corresponding gene. All values are the averages of two independent determinations.