TABLE 4.
Strain | β-Galactosidase activity (nmol/min/mg of protein)
|
|
---|---|---|
hemZ::lacZ | hemN::lacZ | |
FNRL | 5.2 ± 2.0 | 52.1 ± 1.8 |
CCOP1FNRL | 5.2 ± 1.0 | 195.2 ± 1.4 |
Strains were grown aerobically by sparging with 30% O2–69% N2–1% CO2 to an OD600 of 0.4 to 0.5. Plasmids pJE3170 and pUI2762 carrying transcriptional fusions hemZ::lacZ and hemN::lacZ, respectively, were used to determine promoter activities of the corresponding gene. All values are the averages of two independent determinations.