TABLE 2.

Growth characteristics of C. glutamicum grown on minimal medium with sodium [1-¹³C]acetate, sodium [1-¹³C]acetate plus unlabeled glucose, or [5-¹³C]glucose as the carbon and energy source

Growth parametera (unit)	Result for growth on minimal medium with:
	120 mM acetate	120 mM acetate + 55 mM glucose	110 mM glucose
Growth rate (h−1)	0.28	0.36	0.32
Biomass yield (g of C [dry wt] · [g of C in substrate]−1)	0.29	0.41	0.41
Acetate consumption rate (nmol · [mg of protein]−1 · min−1)	540	270
Glucose consumption rate (nmol · [mg of protein]−1 · min−1)		72	148
Carbon consumption rate (nmol of C · [mg of protein]−1 · min−1)	1,080	972	888

^aErrors were <5% for determinations of growth rates, <5% for determinations of biomass yields, and <10% for determinations of carbon consumption rates. These data coincided with values obtained from at least three independent cultures with unlabeled substrates.