Figure 3.

Effect of dimethyl fumarate (DMF), standard treatment and standard treatment with DMF on Mtb loads in the infected spleens after 8 weeks of treatment. (A) Spleen homogenates were used for CFU and MPN_CSN assays. Data shown as means ± STDV (N = 5) (B). The resuscitation indices were calculated using the following formula: RI= log₁₀ (MPN_CSN ml^-1) – log₁₀ (CFU ml^-1). Ns, non-significant; p > 0.05; *p < 0.05; ****p < 0.0001 unpaired t-test. No Mtb were recovered in the DHZR group; the presented bars indicate the limit of detection for the MPN assay 4.6 cell ml^-1 and 0.25 cell ml^-1 (CFU). PEG, control PEG 400 group; DMF, dimethyl fumarate group; RHZ, rifampicin, isoniazid and pyrazinamide group; and DRHZ, rifampicin, isoniazid, pyrazinamide and dimethyl fumarate group.