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Expression of lactoferrin (LTF) before and after cryopreservation. Western blotting analysis of protein expression in fresh and freeze-thawed ram sperm (A). Relative expression levels of LTF in fresh and frozen–thawed sperm (B). Values represent the average± standard error of the mean from three gels per group. Asterisks indicate a statistically significant difference from the fresh group: ** p<0.001 from one-way analysis of variance with Duncan’s post hoc analysis.
