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. 2022 Sep 5;31:09636897221116085. doi: 10.1177/09636897221116085

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — The expression of ATF7 was correlated with the expression of Pin1. (A-B) Western blot analysis was used to detect the expression of ATF7 in Pin1-knockdown or Pin1-overexpressed CNE1 cells. The relative expression levels of Pin1 and ATF7 were quantified (n = 4 independent repeats). (C) Whole-mount embryo immunofluorescence staining of ATF7 expression in tumor cells transplanted in zebrafish. Images of selected areas (white dashed line) are shown at higher magnification below. Scale bar: 50 μm; 10 μm in the amplified image. The mean fluorescence intensity of Pin1 and ATF7 was analyzed quantitatively. The data were quantitatively analyzed by Student’s t test (CNE1-LV3 group, n = 5 transplanted fishes analyzed; CNE1-shPin1 group, n = 3 transplanted fishes analyzed). Data are shown as mean ± SD. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; EGFP: enhanced green fluorescent protein. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.