Table 1.

Different extraction methods For alkaloids and obtained alkaloid fraction through each one of them (amount was calculated as a ratio between the peak area (mAU) and the concentration of the sampe (g/mL) multiplied by 10⁵.

	General extraction	Alkaloid extraction	Alkaloid micro-extraction
Sample preparation	- Dissolve the evaporated sample in 20.0 mL methanol - Filter through a 0.45 μm filter before injecting	- Dissolve the evaporated sample in 15.0 mL 5% acetic acid - Perform three times liquid-liquid extraction with 10.0 mL hexane - Prepare an aqueous solution alkaline to pH 9.0 with ammonia - Perform three times liquid-liquid extraction with 10.0 mL chloroform - Evaporate the combined chloroform fractions - Dissolve in 20.0 mL methanol - Filter through a 0.45 μL filter before injecting	- Dissolve the evaporated sample in 10.0 mL 5% acetic acid - Transfer into a headspace vial, add 8.0 mL of hexane and close the vial - Shake for 15 min in an ultrasonic bath in a horizontal position - Remove the hexane layer and make the aqueous layer alkaline to pH 9.0 with ammonia - Add 8.0 mL of chloroform and shake for 30 min in an ultrasonic bath in a horizontal position - Repeat two more times - Evaporate the combined chloroform fractions - Dissolve in 20.0 mL methanol - Filter through a 0.45 μL filter before injecting
Obtained alkaloid fraction	4.1373	3.8364	0.3427