Figure 1. Deletion of SOCE in CD4⁺ T cells suppresses the induction of colitis in mice.

• A–E
  Naïve CD4⁺ T cells were isolated from the spleen and LNs of WT, Stim2 ^fl/fl Cd4Cre (S2), Orai1 ^fl/fl Cd4Cre (O1), and Stim1 ^fl/fl Cd4Cre (S1) mice and differentiated into Th1, Th17, and iTreg cells. (A) Fura‐2 loaded cells were stimulated with 0.3 μM ionomycin to induce SOCE. Representative Ca²⁺ traces (left) and peak Ca²⁺ levels (right). Data are from the mean ± SEM of four mice per group. (B) Induction of colitis in Rag1 ^−/− mice by adoptive transfer of naive CD4⁺ T cells from wildtype, Stim2 ^fl/fl Cd4Cre, Orai1 ^fl/fl Cd4Cre and Stim1 ^fl/fl Cd4Cre mice. Weight curves of recipient Rag1 ^−/− mice following injection of naive CD4⁺ T cells. Data are from two independent experiments with eight recipient mice per group. (C) H&E stains of colon tissues and histological inflammation scores of Rag1 ^−/− mice 8 weeks after T cell transfer. Data are from one representative experiment with five mice per group. (D, E) Representative flow cytometry plots and frequencies of donor CD4⁺ T cells producing IFNγ, IL‐17A, TNF or expressing Foxp3 that were isolated from the mesenteric LNs of recipient Rag1 ^−/− mice. Data are from two independent experiments with 3–8 mice per group. Statistical analyses by unpaired student's t‐test: ***P < 0.001 **P < 0.01 *P < 0.05.

Source data are available online for this figure.