Figure 4. Inhibition of SOCE suppresses the activation and function of human colonic T cells.

• A
  FlowSOM plot of merged FCS files from samples treated with PMA/ionomycin ± 1 μM BTP2 (non‐inflamed: n = 4, CD: n = 6, UC: n = 6). Colors of FlowSOM plot indicate 20 distinct clusters of CD45⁺CD3⁺ LPMCs. Heatmaps show the expression levels of 24 markers used for cluster determination. The table shows the mean ± SEM of frequencies (%) for each cell subset defined in non‐inflamed controls, UC and CD patients after 4 h stimulation with PMA/ionomycin in vitro. Statistical significance was calculated using the edgeR statistical framework with negative binomial GLM and a false discovery rate adjusted to 10% using the Benjiamini–Hochberg procedure.
• B
  Box plots showing frequencies (%) of each cell subset defined by the cluster analysis in non‐inflamed, UC and CD samples following stimulation with PMA/ionomycin ± BTP2 for 4 h in vitro. Statistical significances were calculated using a one‐tailed paired Wilcoxon matched‐pairs signed‐rank test, *P < 0.05. Boxes range from the 25^th to 75^th percentiles. Whisker plots show the min (smallest) and max (largest) values. The line in the box denotes the median obtained from one experiment (non‐inflamed: n = 4, CD: n = 6, UC: n = 6 patients).
• C
  viSNE plots of one exemplary CD patient. Identification of cell subsets (left) and expression levels of cytokines after PMA/ionomycin stimulation in the presence or absence of BTP2 (blue; low, red: high).
• D
  Heatmaps indicating the median fold change expression of cytokines and surface molecules in CD45⁺CD3⁺ LPMCs stimulated with PMA/ionomycin ± BTP2 for 4 h in vitro. Values are normalized to stimulated, non‐BTP2 treated samples. Statistical significance was calculated using a one‐tailed paired Wilcoxon matched‐pairs signed‐rank test, *P < 0.05.

Source data are available online for this figure.