Figure 3.

Glycolysis and OxPhos remain largely stable over the course of Chlamydia trachomatis infection in epithelial cells.A, A2EN cells were infected with CTDm for 24, 30, and 48 h followed by ECAR measurements as described in the Experimental procedures. Note that the increase in ECAR observed 48 hpi was no longer significant after Benjamini Hochberg adjustment of the p values, see Table S1 for details. B, experimental setup for the measurement of lactate concentration in the culture medium. C, primary cells (C) and End1 cells (D and E) were infected with CTDm for 24, 30, and 48 h after what the concentration of lactate in the culture medium was measured, without (C and D) or with (E) normalization to the cell number. Primary cells (F and G) and A2EN cells (H and I) were infected with CTDm for 24, 30, and 48 h followed by OCR and spare respiratory capacity measurements as described in the Experimental procedures (see also Fig. S3B). For each panel, the results of 3 or 4 independent experiments and the mean are displayed. Significance is defined as: ns (not significant) p > 0.05; (∗∗∗) = p ≤ 0.001. CTD, C. trachomatis serovar D; ECAR, extracellular acidification rate; hpi, hours postinfection; OCR, oxygen consumption rate; OxPhos, oxidative phosphorylation.