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. 2022 Aug 2;298(9):102338. doi: 10.1016/j.jbc.2022.102338

Figure 5.

Figure 5

Reliance of Chlamydia trachomatis on host glycolysis is by-passed in 2 tumor-derived cell lines.A, HeLa cells were infected with CTDm and treated with indicated concentrations of GNE-140 for 48 h before a progeny assay was performed as described in the Experimental procedures, and IFU was determined. The results of 4 independent experiments and the mean are displayed. B, HeLa cells were infected at MOI 10 with CTDm and treated or not with GNE-140. Cells were fixed 6 hpi, then permeabilized before immunostaining with anti-Cap1 followed with Alexa488-conjugated secondary antibodies. DNA is stained with Hoechst. C, GPI was silenced in HeLa cells with 2 siRNA for 48 h before cells were infected with CTDm. 30 hpi, a progeny assay was performed, and IFU was determined. The results of 4 independent experiments and the mean are displayed. D, efficiency of siRNA treatment was evaluated through a lactate assay 48 h following siRNA treatment in HeLa cells. The results of 2 independent experiments and the mean are displayed. E, LS174T WT or GPI-KO (clone #26 and #8) were infected with CTDm for 48 h before performing a progeny assay and determining IFU. The results of 3 independent experiments and the mean are displayed. CTD, C. trachomatis serovar D; IFU, infection forming unit; hpi, hours postinfection.