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. Author manuscript; available in PMC: 2022 Sep 7.
Published in final edited form as: Cell Rep. 2022 May 17;39(7):110814. doi: 10.1016/j.celrep.2022.110814

Figure 6. STING and STAT1 mediate induction of type I and type II interferons following WEE1 inhibition.

Figure 6.

(A and B) Knockdown of STAT1 (or siRNA control [siSCR]) by siRNA followed by treatment with 1 μM AZD1775 for 48 h in H82 cells.

(A) Western blots show expression of p-WEE1, total STAT1, and actin (loading control).

(B) Quantitative mRNA expression of IFN-α, IFN-β, and IFN-γ.

(C and D) Knockdown of STING (or siSCR) by siRNA followed by treatment with 1 μM AZD1775 for 48 h in H82 cells. The data shown represent the means ± SD of triplicate. p values were calculated by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001).

(C) Western blots showing expression of p-WEE1, total STING, and actin (loading control).

(D) Quantitative mRNA expression of IFN-α, IFN-β, and IFN-γ. The data shown represent the means ± SD of triplicate. p values were calculated by Student’s t test (**p < 0.01, ***p < 0.001).

(E) Quantitative mRNA expression of IFN-α, IFN-β, IFN-γ, CXCL10, and CCL5 after treatment with 500 nM H151 or 1 μM C176 and 1 μM AZD1775 for 48 h in H526 cells. The data shown represent the means ± SD of triplicate. p values were calculated by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001).

See also Figure S8.