KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Phospho-Wee1 (Ser642) | Cell Signaling Technology | Cell Signaling Technology Cat# 4910, RRID:AB_2215870 |
| STING (D2P2F) | Cell Signaling Technology | Cell Signaling Technology Cat# 13647, RRID:AB_2732796 |
| Phospho-STING (Ser366) | Cell Signaling Technology | Cell Signaling Technology Cat# 19781, RRID:AB_2737062 |
| TBK1 | Cell Signaling Technology | Cell Signaling Technology Cat# 3504, RRID:AB_2255663 |
| phospho-TBK1(S172) | Cell Signaling Technology | Cell Signaling Technology Cat# 5483, RRID:AB_10693472 |
| Stat1 | Cell Signaling Technology | Cell Signaling Technology Cat# 14994, RRID:AB_2737027 |
| Phospho-Stat1 (Ser727) | Cell Signaling Technology | Cell Signaling Technology Cat# 8826, RRID:AB_2773718 |
| IRF-3 | Cell Signaling Technology | Cell Signaling Technology Cat# 11904, RRID:AB_2722521 |
| Phospho-IRF-3 (Ser386) | Cell Signaling Technology | Cell Signaling Technology Cat# 37829, RRID:AB_2799121 |
| Phospho-Histone H2A.X (Ser139) | Cell Signaling Technology | Cell Signaling Technology Cat# 2577, RRID:AB_2118010 |
| CDK1 (cdc2) | Cell Signaling Technology | Cell Signaling Technology Cat# 77055, RRID:AB_2716331 |
| Phospho-CDK1 (cdc2) (Tyr15) | Cell Signaling Technology | Cell Signaling Technology Cat# 4539, RRID:AB_560953 |
| cGAS | Cell Signaling Technology | Cell Signaling Technology Cat# 15102, RRID:AB_2732795 |
| Histone H3 | Cell Signaling Technology | Cell Signaling Technology Cat# 4499, RRID:AB_10544537 |
| Lamin B1 | Cell Signaling Technology | Cell Signaling Technology Cat# 12586, RRID:AB_2650517 |
| Cleaved PARP (Asp214) | Cell Signaling Technology | Cell Signaling Technology Cat# 5625, RRID:AB_10699459 |
| Cleaved PARP (Asp214) | Cell Signaling Technology | Cell Signaling Technology Cat# 94885, RRID:AB_2800237 |
| PD-L1 | Cell Signaling Technology | Cell Signaling Technology Cat# 13684, RRID:AB_2687655 |
| β-Actin | Cell Signaling Technology | Cell Signaling Technology Cat# 4967, RRID:AB_330288 |
| Anti-rabbit IgG, HRP-linked Antibody | Cell Signaling Technology | Cell Signaling Technology Cat# 7074, RRID:AB_2099233 |
| CD4-APC Cy7 | Biolegend | BioLegend Cat# 100526, RRID:AB_312727 |
| CD8-PE Cy7 | Biolegend | BioLegend Cat# 100722, RRID:AB_312761 |
| CD45-Pacific Blue | Biolegend | BioLegend Cat# 103126, RRID:AB_493535 |
| CD4-PE/Dazzle-594 | Biolegend | BioLegend Cat# 100246, RRID:AB_2565883 |
| Ghost Dye™ Violet 510 | Tonbo | Cat#13-0870-T500 |
| PD-1-BV605 | Biolegend | BioLegend Cat# 135220, RRID:AB_2562616 |
| CD62L-AF700 | BD Biosciences | BD Biosciences Cat# 560517, RRID:AB_1645210 |
| CD44-BV711 | Biolegend | BioLegend Cat# 103057, RRID:AB_2564214 |
| Tim-3-APC | Biolegend | BioLegend Cat# 134008, RRID:AB_2562998 |
| GR-1-BV711 | Biolegend | BioLegend Cat# 108443, RRID:AB_2562549 |
| F/80-APC | Tonbo | Tonbo Biosciences Cat# 20-4801, RRID:AB_2621602 |
| CD11b-BV650 | Biolegend | BioLegend Cat# 101259, RRID:AB_2566568 |
| CD68-PerCP/Cy5.5 | Biolegend | BioLegend Cat# 137010, RRID:AB_2260046 |
| iNOS-PE | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# 12-5920-80, RRID:AB_2572641 |
| PD-L1-PE | BD Biosciences | BD Biosciences Cat# 558091, RRID:AB_397018 |
| PD-L1-APC | Biolegend | BioLegend Cat# 329708, RRID:AB_940360 |
| H-2Kb/H-2Dd-FITC | Biolegend | BioLegend Cat# 114706, RRID:AB_313605 |
| HLA-ABC-PE | Biolegend | BioLegend Cat# 311406, RRID:AB_314875 |
| CD3 | Abcam | Abcam Cat# ab135372, RRID:AB_2884903 |
| CD8a | Synaptic Systems | Synaptic Systems Cat# HS-361 003 |
| PD-L1 | R and D Systems | R and D Systems Cat# AF1019, RRID:AB_354540 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| AZD1775 | Selleck chemical | Cat# S1525 |
| H151 | Selleck chemical | Cat# S6652 |
| C176 | Selleck chemical | Cat# S6575 |
| Anti-PD-L1 antibodiy (clone 10F.9G2) | Bio X cell | Bio X Cell Cat# BE0101, RRID:AB_10949073 |
| IgG2b I sotype control | Bio X cell | Bio X Cell Cat# BE0090, RRID:AB_1107780 |
| Cisplatin | Accord Healthcare | Cat# 16729-288-38 |
| Etoposide | Accord Healthcare | Cat# 16729-114-08 |
| Recombinant Human IFN-γ | BioLegend | Cat# 570204 |
|
| ||
| Critical commercial assays | ||
|
| ||
| CellTiter-Glo luminescent cell viability assay | Promega | Cat# G7571 |
| Annexin V-propidium iodide (PI) assay |
BD Biosciences | BD Biosciences Cat# 556547, RRID:AB_2869082 |
| Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit |
Thermo Fisher | Cat# C10425 |
| ProLong Gold Antifade Mountant with DAPI |
Cell Signaling Technology | Cat# 8961S |
|
| ||
| Deposited data | ||
|
| ||
| Raw RNAseq data | This paper | ArrayExpress: E-MTAB-11577 |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| NCI-H1048 | ATCC | ATCC Cat# CRL-5853, RRID:CVCL_1453 |
| NCI-H196 | ATCC | ATCC Cat# CRL-5823, RRID:CVCL_1509 |
| NCI-H211 | ATCC | ATCC Cat# CRL-5824, RRID:CVCL_1529 |
| NCI-H82 | ATCC | ATCC Cat# HTB-175, RRID:CVCL_1591 |
| NCI-H446 | ATCC | ATCC Cat# HTB-171, RRID:CVCL_1562 |
| NCI-H1341 | ATCC | ATCC Cat# CRL-5864, RRID:CVCL_1463 |
| NCI-H720 | ATCC | ATCC Cat# CRL-5838, RRID:CVCL_1583 |
| NCI-H524 | ATCC | ATCC Cat# CRL-5831, RRID:CVCL_1568 |
| NCI-H841 | ATCC | ATCC Cat# CRL-5845, RRID:CVCL_1595 |
| NCI-H526 | ATCC | ATCC Cat# CRL-5811, RRID:CVCL_1569 |
| NCI-H1694 | ATCC | ATCC Cat# CRL-5888, RRID:CVCL_1489 |
| NCI-H146 | ATCC | ATCC Cat# HTB-173, RRID:CVCL_1473 |
| DMS 114 | ATCC | ATCC Cat# CRL-2066, RRID:CVCL_1174 |
| COR-L311 | ECACC | ECACC Cat# 96020721, RRID:CVCL_2412 |
| NCI-H69 | ATCC | ATCC Cat# HTB-119, RRID:CVCL_1579 |
| DMS 79 | ATCC | ATCC Cat# CRL-2049, RRID:CVCL_1178 |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| Athymic nu/nu, females, 6 weeks old | ENVIGO | Stock #: 069 |
| B6 129F1, females, 6 weeks old | TACONIC FARMS INC. | Stock #: B6129-F |
| B6 FVBF1/J, females, 6 weeks old | JACKSON LABORATORY | Stock #: 019019 |
| NSG, females, 6 weeks old | JACKSON LABORATORY | Stock #: 005557 |
|
| ||
| Software and algorithms | ||
|
| ||
| FlowJo Ver.10 | BD Biosciences | https://www.flowjo.com |
| Prism Ver. 9.0 | Graphpad | N/A |
| Case Viewer | 3DHISTECH | https://www.3dhistech.com/solutions/caseviewer/ |