Table 3.
Effect of eggshell temperature (EST) during late incubation (≥embryonic d 17; 37.8°C [control] or 36.7°C [lower]) and/or feeding strategy after hatch (direct access to feed and water [early] or 51–54 h deprivation [delayed]) on the number of attempts or vocalizations (mean ± SE) and latency to attempt to stand up (LSmean ±SEM) of broilers during a tonic immobility test at d 13.
| Treatment | n1 | Attempts (no.) | Vocalizations (no.) | Latency (sec) |
|---|---|---|---|---|
| EST | ||||
| Control | 16 | 3 ± 0.3 | 5 ± 2.0 | 139 |
| Lower | 16 | 2 ± 0.2 | 6 ± 1.9 | 170 |
| SEM | 27.4 | |||
| Feeding strategy | ||||
| Delayed | 16 | 2 ± 0.2 | 6 ± 2.1 | 161 |
| Early | 16 | 3 ± 0.3 | 5 ± 1.8 | 148 |
| SEM | 27.4 | |||
| EST × Feeding strategy | ||||
| Control × Delayed | 8 | 2 ± 0.4 | 7 ± 2.7 | 125 |
| Control × Early | 8 | 3 ± 0.5 | 4 ± 2.9 | 154 |
| Lower × Delayed | 8 | 3 ± 0.3 | 5 ± 3.2 | 202 |
| Lower × Early | 8 | 2 ± 0.2 | 6 ± 2.1 | 143 |
| SEM | 38.5 | |||
| P-values | ||||
| EST | 0.28 | 0.72 | 0.62 | |
| Feeding strategy | 0.69 | 0.78 | 0.96 | |
| EST × Feeding strategy | 0.15 | 0.09 | 0.99 | |
1
Pens, with 1 male and 1 female broiler / pen.