TABLE 3.

Relative activities of 4-nitrobenzyl alcohol dehydrogenase^a

Substrate	Source of cell extract
	TW3 grown on 4-nitrotoluene	E. coli BL21(DE3) expressing ntnD
4-Nitrobenzyl alcohol	100	100
3-Nitrobenzyl alcohol	30	33
2-Nitrobenzyl alcohol	3	3
4-Methylbenzyl alcohol	117	126
3-Methylbenzyl alcohol	98	120
2-Methylbenzyl alcohol	14	24
4-Hydroxybenzyl alcohol	3	5
3-Hydroxybenzyl alcohol	104	110
2-Hydroxybenzyl alcohol	5	18
2,4-Dimethylbenzyl alcohol	28	36
2,5-Dimethylbenzyl alcohol	2	2
3,4-Dimethylbenzyl alcohol	140	148
3,5-Dimethylbenzyl alcohol	70	63
4-Ethylbenzyl alcohol	114	113
Benzyl alcohol	121	108
l-Sorbose, decanol, octanol, hexanol, butanol, ethanolb	2	2

^aActivities are expressed as percentages relative to that observed with 4-nitrobenzyl alcohol, each value being the mean from two different cultures. No individual value was more than ± 3% from the mean. 

^bAll of these aliphatic alcohols stimulated the same low activities.