Table 1:

Antibody Comparisons & Characteristics in Hematological Malignancies

Antibody Clones	T-Cell	T-ALL	B-Cells	B-ALL	CD34+	AML	MDS	Subtype	Target Species	Epitopes
DN16	Co-stimulation along with suboptimal CD3 mAb OKT3 enhanced proliferation of T-cells and stimulation of IL-2.52	Induction of HSP70 in Jurkat cells.44 Induced homotypic cell aggregation of Jurkat T-cells and cell apoptosis.45	Induction of cell death of undifferentiated B-cell precursors.46	Induction of HSP70 in REH cells.44 Induces cell apoptosis of BCP-ALL.46				IgG	Mouse (reacts with Human)	Recognizes ‘LPDNENKK’ sequence located between residues 32 and 39 towards the N-terminus.56
YG32		Induced homotypic aggregation of Jurkat T-cells but no cell apoptosis. However, it enhanced Fas-mediated cell death.45						IgG	Mouse	Recognizes ‘RPPNPPK’ sequence between residues 68 and 74.56
12E7	Does not induce cell death of thymocytes.10			Does not induce cell apoptosis.46	Slight protection from apoptosis, inhibits transendothelial migration of CD34+ and homing to bone marrow.53			IgG1	Mouse	Recognizes ‘DGEN’ sequence between residues 61~64.28
0062	Inhibit T-cell rosette formation. Induced cell death of thymocytes.10	Induced cell death of Jurkat T-cells.10	Induction of cell death of undifferentiated B-cell precursors.46	Induction of HSP70 in REH cells.44 Does not induce cell apoptosis.46	Does not induce apoptosis and has no effect on cell migration.53				Human	Recognizes ‘GSFSDADLAD’ sequence between residues 88~97.28
TU12	Enhanced CD4+ cell proliferation in the presence of CD3 costimulation.54							IgG2	Mouse
Hec2				Induction of HSP70 in REH cells.44	Does not induce apoptosis and has no effect on cell migration.53
3B2/TA8	Enhanced proliferation of peripheral blood T-cells along with CD3 mAb.53	Increased IL-2 promoter activity in Jurkat cells in the presence of CD3 mAb.53		Does not induce cell apoptosis.46				IgG2	Mouse
AD20		Induces cell aggregation and apoptosis in Jurkat T-cells.55						IgM	Human
HO36-1.1		Induces apoptosis in Jurkat T-cells.55				Cytotoxic to AML cell lines and myeloid blasts.19	Cytotoxic to purified primary MDS CD34+ cells.19	IgM	Mouse

Antibody Comparisons in Hematological Malignancies
Antibody Clones	T-Cell	T-ALL	B-Cells	B-ALL	CD34+	AML	MDS
DN16	Co-stimulation along with suboptimal CD3 mAb OKT3 enhanced proliferation of T-cells and stimulation of IL-2.52	Induction of HSP70 in Jurkat cells.44 Induced homotypic cell aggregation of Jurkat T-cells and cell apoptosis.45	Induction of cell death of undifferentiated B-cell precursors.46	Induction of HSP70 in REH cells.44 Induces cell apoptosis of BCP-ALL.46
YG32		Induced homotypic aggregation of Jurkat T-cells but no cell apoptosis. However, it enhanced Fas-mediated cell death.45
12E7	Does not induce cell death of thymocytes.10			Does not induce cell apoptosis.46	Slight protection from apoptosis, inhibits transendothelial migration of CD34+ and homing to bone marrow.53
0062	Inhibit T-cell rosette formation. Induced cell death of thymocytes.10	Induced cell death of Jurkat T-cells.10	Induction of cell death of undifferentiated B-cell precursors.46	Induction of HSP70 in REH cells.44 Does not induce cell apoptosis.46	Does not induce apoptosis and has no effect on cell migration.53
TU12	Enhanced CD4+ cell proliferation in the presence of CD3 costimulation.54
Hec2				Induction of HSP70 in REH cells.44	Does not induce apoptosis and has no effect on cell migration.53
3B2/TA8	Enhanced proliferation of peripheral blood T-cells along with CD3 mAb.53	Increased IL-2 promoter activity in Jurkat cells in the presence of CD3 mAb.53		Does not induce cell apoptosis.46
AD20		Induces cell aggregation and apoptosis in Jurkat T-cells.55
HO36-1.1		Induces apoptosis in Jurkat T-cells.55				Cytotoxic to AML cell lines and myeloid blasts.19	Cytotoxic to purified primary MDS CD 34+ cells19

Antibody Characteristic Comparisons
Antibody	Subtype	Target Species	Epitopes
DN16	IgG	Mouse (reacts with Human]	Recognizes ‘LPDNENKK’ sequence located between residues 32 and 39 towards the N-terminus.56
YG32	IgG	Mouse	Recognizes ‘RPPNPPK’ sequence between residues 68 and 74.56
12E7	IgG1	Mouse	Recognizes ‘DGEN’ sequence between residues 61~64.28
0062		Human	Recognizes ‘GSFSDADLAD’ sequence between residues 88~97.28
TU12	IgG2	Mouse
3B2/TA8	IgG2	Mouse
AD20	IgM	Human
HO36-1.1	IgM	Mouse