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. 2022 Aug 28;8(9):e10416. doi: 10.1016/j.heliyon.2022.e10416

Figure 7.

Figure 7

PLAAT4 Knockdown attenuated the Proliferation Ability of Pancreatic Cancer Cells. (A) The expression of PLAAT4 in pancreatic cancer cell lines (HPNE, CFPAC-1, AsPC-1, BxPC-3, Panc-1, PATU8988, MIA PaCa-2) were separately measured by western blotting. (B) The expression of PLAAT4 in pancreatic cancer cell lines (HPNE, CFPAC-1, AsPC-1, BxPC-3, Panc-1, PATU8988, MIA PaCa-2) were measured by qPCR. (C) The efficiency of si-PLAAT4 in AsPC-1 and BxPC-3 cell lines were tested by western blotting. (D) The efficiency of PLAAT4-overexpression in AsPC-1 and MIA PaCa-2 cell lines were verified by western blotting. (E) The CCK-8 assay was used to detect the proliferation ability after si-PLAAT4 in AsPC-1 and BxPC-3 cell lines. (F) The CCK-8 assay was used to detect the proliferation ability after PLAAT4-overexpression in AsPC-1 and MIA PaCa-2 cell lines. Representational images of the colony formation assay after si-PLAAT4 in AsPC-1 and BxPC-3 cell lines. (G–J) Digitization of the colony formation assay after si-PLAAT4 in AsPC-1 and BxPC-3 cell lines and after PLAAT4-overexpression in AsPC-1 and MIA PaCa-2 cell lines. (K–N) Digitization of the results of cell cycle assay after si-PLAAT4 in AsPC-1 and BxPC-3 cell lines and after PLAAT4-overexpression in AsPC-1 and MIA PaCa-2 cell lines. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.