TABLE 1.
List of E. coli strainsa
| Strain | Genotypeb | Source or reference |
|---|---|---|
| BL21 | hsdS gal | 33 |
| JL2301c | thr leu arg his ΔlexA300::(Smr Spr) sulA (λsulA::lac) | J. Little via M. Radman |
| MG1655 | Wild type | Lab stock |
| SG20780 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 Δlon-510 cpsB10::lac-Mu-λ-imm | 57 |
| SG20781 | SG20780 lon+ | 57 |
| GJ1823 | MG1655 zbh-900::Tn10dKan(Ts)1 lacZ4525::Tn10dKan lon-103::IS186 | 44 |
| GJ1901 | ara zbh-900::Tn10dKan(Ts)1 lacZ(Am) lacI3098::Tn10Kan | From CSH142 (33), in several steps |
| GJ1904 | GJ1901 uvrD400::Tn10dTet | This study |
| GJ1907 | MG1655 zbh-900::Tn10dKan(Ts)1 lon-103::IS186 | This study |
| GJ1912 | MG1655 lon-103::IS186 zba-901::Tn10dKan | This study |
| GJ1913 | MG1655 zba-901::Tn10dKan | This study |
| GJ1916 | MG1655 lon-104::IS186::Tn10dCm zba-901::Tn10dKan | This study |
| GJ1922 | JL2301 lexA+ malB::Tn9 | This study |
| GJ1925 | JL2301 lexA+ malF3180::Tn10Kan | This study |
| GJ1988 | GJ1907 sulA::Tn5 uvrD400::Tn10dTet | This study |
| GJ1989 | GJ1907 lexA3 malB::Tn9 uvrD400::Tn10dTet | This study |
| GJ1992 | JL2301 lexA+ | This study |
| GJ1997 | GJ1907 sulA::Tn5 dam::Tn9 | This study |
| GJ1998 | GJ1907 sulA::Tn5 dnaQ (mutD5) zae-502::Tn10 | This study |
| GJ1999 | GJ1907 lexA3 malB::Tn9 priA1::Kan | This study |
| GJ2101 | SG20780 ΔuvrD288::Kan/pHYD141 | This study |
| GJ2102 | SG20780 ΔuvrD288::Kan recA56 srl-300::Tn10 | This study |
| GJ2122 | MG1655 ΔpolA::Kan lon-146::Tn10dTet/F′[polA(Klenow+)Cmr] | This study |
| GJ2126 | SG20780 uvrD3 ilv-500::Tn10 | This study |
| GJ2127 | SG20780 Δrep::Cm | This study |
Strain BL21 is an E. coli B derivative. All other strains are derivatives of E. coli K-12. Genotypes of additional sulA-lac strains used in this study are described in Table 2.
Genotype designations are as in the work of Berlyn (2). All strains are F− unless otherwise indicated. Allele numbers are given where they are known. Listed below in parentheses are references or sources for various mutations introduced into the GJ strains: ilv-500::Tn10, lacI3098::Tn10Kan, and malF3180::Tn10Kan (52); lacZ(Am), dnaQ, and zae-502::Tn10 (33); ΔpolA::Kan and F′[polA(Klenow+)Cmr] (12); lon-146::Tn10dTet (31); dam::Tn9 (N. Kleckner); ΔuvrD288::Kan (56); priA1::Kan (36); lexA3, sulA::Tn5, and malB::Tn9 (M. Radman); Δrep::Cm (B. Michel); recA56 and srl-300::Tn10 (44); and uvrD3 (E. coli Genetic Stock Center).
Genotype markers of JL2301 relevant to this study are listed.