TABLE 2.
Effects of mutations on sulA::lac expressiona
| Strain | Mutationb | Enzyme sp act |
|---|---|---|
| GJ1922 derivatives | ||
| GJ1922 | None | 49 |
| With pHYD136 | 44 | |
| GJ1923 | ΔlexA300::(Smr Spr) | 3,574 |
| GJ1927 | dnaQ (mutD5) zae-502::Tn10 | 138 |
| GJ1930 | uvrD400::Tn10dTet | 264 |
| Grown at 42°C | 236 | |
| GJ1931 | priA1::Kan | 397 |
| GJ1965 | ΔuvrD288::Kan | 258 |
| GJ1966 | uvrD3 ilv-500::Tn10 | 36 |
| GJ1991 | lexA3 malF3180::Tn10Kan uvrD400::Tn10dTet | 23 |
| GJ2103 | mutH471::Kan | 51 |
| GJ2104 | mutS::Tn10dTet | 62 |
| GJ2105 | uvrC279::Tn10 | 59 |
| GJ2106 | mutH471::Kan uvrC279::Tn10 | 65 |
| GJ2107 | mutS::Tn10dTet ΔuvrD288::Kan | 239 |
| GJ2108 | uvrC279::Tn10 ΔuvrD288::Kan | 271 |
| GJ1925 derivatives | ||
| GJ1925 | None | 52 |
| GJ1929 | dam::Tn9 | 237 |
| GJ1992 derivatives | ||
| GJ1992 | None | 52 |
| GJ1993 | ΔpolA::Kan | 2,050 |
| GJ1994 | ΔpolA::Kan/F′ [polA+ Cmr] | 37 |
| GJ1995 | ΔpolA::Kan/F′ [polA(Klenow+)Cmr] | 1,488 |
| With IPTG | 41 | |
| GJ1996 | ΔpolA::Kan/F′ [polA(5′ Exo+)Cmr] | 197 |
a
The specific activity of β-galactosidase was determined in each of the cultures grown to exponential phase in glucose-minimal medium (for strains GJ1993 and GJ1995) or LB (for all other strains), and values (representing the means of at least three independent experiments) are given in Miller units (33).
b
Mutations in addition to those present in the parental strain GJ1922, GJ1925, or GJ1992 are listed for each derivative. The reference or source for each of the mutations not described in Table 1 is given below in parentheses: mutH471::Kan (E. coli Genetic Stock Center); mutS::Tn10dTet (33); uvrC279::Tn10 (52); F′[polA+ Cmr] and F′[polA (5′ Exo+)Cmr] (12).