Figure 2.

GPR35 surface expression and project overview.A, sequence alignment of the variable, N-terminal regions of GPR35 short and long. B, comparison of receptor expression of both GPR35 isoforms at the surface of HEK293 cells. Data shown present mean ± s.e.m. of four independent experiments. Statistical significance was tested using One-way ANOVA followed by Tukey’s multiple comparison; p < 0.05. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. The negative control (pcDNA transfection) was normalized to 0, and the expression of GPR35 short was set to 1.0 for each individual experiment. C, overview of the employed GPR35 ligands and intracellular signaling pathways monitored in the present study. The signaling components highlighted in red were detected in living cells using different optical biosensors. HEK293, human embryonic kidney 293A.