Figure 3.
Constitutive activation of heterotrimeric G proteins by GPR35.A–J, normalized BRET0 values of the indicated G protein sensors cotransfected with pcDNA or GPR35 isoforms. K–N, transcriptional activity in cells cotransfected with G protein–dependent reporter gene plasmids along with pcDNA or GPR35 isoforms. Data show mean ± s.e.m. of nine (A–J) or three (K–N) independent experiments conducted in transiently transfected HEK293A cells. Statistical significance was tested using One-way ANOVA followed by Tukey’s multiple comparison; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. BRET, bioluminescence resonance energy transfer; HEK293, human embryonic kidney 293A.