Figure 4.

Ligand-induced, GPR35-mediated activation of heterotrimeric G protein biosensors. Concentration response curves of pamoic acid, kynurenic acid, and zaprinast obtained using BRET-based biosensors of G_i1 (A–C), G_i2 (D–F), G_i3 (G–I), G_o1 (J–L), G_z (M–O), G_q (P–R), G₁₅ (S–U), G_s (V–X), G₁₂ (y–aa), and G₁₃ (ab–ad). Data show mean ± s.e.m. of three to four independent experiments conducted in transiently transfected HEK293A cells. Vehicle-treated ΔBRET values were set to 0. BRET, bioluminescence resonance energy transfer; HEK293, human embryonic kidney 293A.