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. 2000 Jun;182(11):3158–3164. doi: 10.1128/jb.182.11.3158-3164.2000

FIG. 1.

FIG. 1

Effect of deletions in SIN3, RPD3, and UME6 genes on cell growth. (A and B) Tenfold serial dilutions of cells were plated and incubated at 30°C for 3 days on M.ammonia–25 μg of uracil. Strains 12T7c (ura3), 12T7cII (ura3 sin3::kanMX4), and 12T7cIII (ura3 rpd3::kanMX4) are isogenic to strain Σ1278b (A). Strains BY4709 (ura3), BY4709II (ura3 sin3::kanMX4), BY4709III (ura3 rpd3::kanMX4), and BY4709I (ura3 ume6::kanMX4) are isogenic to S288c (B). (C) Tetrad analysis of the Σ1278b isogenic diploid strain 27061b (ura3 trp1) × 27029c (ura3 leu2) in which one copy of the UME6 gene was replaced by the kanMX4 cassette. Spores were isolated on YPD medium. WT, wild type.