Table 1.
Sequences of primers used in qPCR
| Gene | Primer Sequence (5′->3′) |
|---|---|
| BAX | F: GGCACCTCTTCCCTCCTTTCT |
| R: CGATGCGCTTGAGACACTCG | |
| BCL2 | F: TTCTTTGAGTTCGGTGGGGT |
| R: GGGCCGTACAGTTCCACAA | |
| p21 | F: GAAGAGAAACCCCCAGCTCC |
| R: TGACTGCATCAAACCCCACA | |
| p53 | F: TACTCCCCTGCCCTCAACAA |
| R: AGGAATCAGGGCCTTGAGGA | |
| IL-1β | F: TATGTGTGTGATGCAGCTGTG |
| R: ACTCAAATTCCACGTTGCCC | |
| IL-6 | F: CGTCACTCCAGTTGCCTTCT |
| R: GCCAGTACCTCCTTGCTGTT | |
| TNF α | F: TCCTACCCGTCCAAGGTCAA |
| R: CTCATACCAGGGCTTGGCTT | |
| IL-10 | F: CTAGGGAACGAAGCATCCAGG |
| R: TCAGGAGAGAGGTACCACAGG | |
| IL-18 | F: CGCACCCCAGACCGTATTTA |
| R: CGCTAGACCTCTAGTGAGGC | |
| CFLAR | F: CCACTTAGGAAACAGCTGCC |
| R: AGGCCTTGAGGCTACTGACT | |
| CRSL1 | F: TGGGCCAGTCAAAAATCAGC |
| R: TTCGCGGTGTTGGAAGAGTT | |
| MALT1 | F: ATGATGTGCGACGCCTATGT |
| R: AGCTGCCAGTTTCTTCAGGT | |
| CARD9 | F: CAGCAACTCCTGTGGCTCAT |
| R: GCACTCGTCCTCGTTCTCAT | |
| NLRP3 | F: CGACACTCTGACATGGACCG |
| R: GGCTGAGAAGAGGCTCTGAC | |
| MAP2K1 | F: ACTGGGAAAGCTTGGGATGC |
| R: CAGTTCCCCAACCTTCTGCT | |
| MAP2K2 | F: CGCAGGACTTCCAGGAGTTT |
| R: CATCTTCAGATCCGCTCGCT | |
| MAPK8 | F: GTTGGGTGCATCATGGGAGA |
| R: CAGGACATGGTGTTCCAAGC | |
| MAPK14 | F: GAACGTTGTTTCCTGGCACA |
| R: TCTTGCAGACTCTGAGGAGATTT | |
| NFKB | F: CTTCCTTCGAGCCAGTGACG |
| R: CCAGGAGACTTGCTGTCGTG | |
| IKK | F: GGCGGTTGACATTAGCACAG |
| R: CTGAAGCCGAACCACAGTCT | |
| NFKBIA | F: CACTTCACCTTCGTGAGGCT |
| R: TGTCACAGGACACAACTGGG | |
| TLR1 | F: GTGAGTGGTGCCATTATGAG |
| R: CGCGTTTAGATTCAGGTC | |
| TLR2 | F: TCGCCGGGACTCTCTTTCTC |
| R: GGCCTTGAGGTTCACACACT | |
| TLR3 | F: GACTGATGCTCCGAAGGGTG |
| R: GGTTTGCGTGTTTCCAGAGC | |
| PGC1A | F: GGCCTTCTAAACGTGGGACA |
| R: CCGGAGGTCTGCCATTTTCT | |
| FIS1 | F: GGTGCGAAGCAAGTACAACG |
| R: GTTGCCCACAGCCAGATAGA | |
| MFN1 | F: AAGTGGCATTTTTCGGCAGG |
| R: TCCATATGAAGGGCATGGGC | |
| MFN2 | F: AGGTGAAGTCAGAATTGGTGGA |
| R: CTTCACAGGGGTGGCATCAT | |
| Parkin | F: TCCCAGTGGAGGTCGATTCT |
| R: CCCTCCAGGTGTGTTCGTTT | |
| PINK1 | F: GCACAATGAGCCAGGAGCTA |
| R: GGGGTATTCACGCGAAGGTA | |
| GAPDH | F: GATGCCCCAATGTTTGTGA |
| R: AAGCAGGGATGATGTTCTGG |
BAX BCL-2-associated X protein, BCL-2 B-cell lymphoma 2, p21 cyclin-dependent kinase inhibitor 1A, p53 tumor suppressor p53, IL-1β Interleukin1β, IL-6 Interleukin 6, TNFα tumor necrosis factor, IL-10 interleukin 10, IL-18 interleukin 18, CFLAR CASP8 and FADD-like apoptosis regulator, CRSL1 cardiolipin synthase 1, MALT1 mucosa-associated lymphoid tissue lymphoma translocation protein 1, CARD9 Caspase recruitment domain-containing protein 9, NLRP3 NLR family pyrin domain containing 3, MAP2K1 dual specificity mitogen-activated protein kinase kinase 1, MAP2K2 mitogen-activated protein kinase kinase 2, MAPK8 mitogen-activated protein kinase 8, MAPK14: mitogen-activated protein kinase 14, NFKB nuclear factor kappa B, IKK IkappaB kinase, NFKBIA nuclear factor kappa-B inhibitor, TLR1 toll-like receptor 1, TLR2 toll-like receptor 2, TLR3 toll-like receptor 3, PGC1A peroxisome proliferator-activated receptor-gamma coactivator, FIS1 mitochondrial fission 1 protein, MFN1 mitofusin-1, MFN2 mitofusin-2, MFF mitochondrial fission factor, PARKIN E3 ubiquitin ligase parkin, PINK1 PTEN induced kinase 1, GAPDH glyceraldehyde 3-phosphate dehydrogenase