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Damaging PR increases PRM transcription initiation (A) and open complex formation at PRM (B) in the absence of the 434 repressor. DNA containing a wild-type PRM and a wild-type (lanes 1) or defective (lanes 2) PR promoter was transcribed by RNA polymerase (A) or incubated with RNA polymerase and footprinted using KMnO4 (B). The positions of the PR and PRM transcripts are indicated, as is the position of the PRM open complex.
