Fig. 5.

circPDK1 acts as a ceRNA to modulate BPTF expression in PC. A Potential target mRNAs of miR-628-3were predicted using mirDIP, RNAInter, miRDB, miRTarBase and TargetScan. B The correction between BPTF and miR-628-3p were determined from TCGA. C Volcano plot of differentially expressed protein-coding genes after the loss of circPDK1. D GSEA results were plotted to visualize the correlation between the expression of circPDK1 and c-myc relative pathway in PANC1 cells. E The correction between BPTF and miR-628-3p were determined from our center. F BPTF mRNA expression level was detected after treated with miR-628-3p mimics and inhibitor. G Luciferase activity in 293-T cells co-transfected with Luc-BPTF wild-type or mutant sequence and miR-628-3p mimics or inhibitor. H The correction between circPDK1 and BPTF were evaluated from our center. I BPTF expression was determined by qRT-PCR in MIA PaCa-2 and PANC-1 cells transfected with indicated treatments. J BPTF, c-myc, CCND1 and p21 expression was evaluated by Western blotting with indicated treatments. K Relative luciferase activity of Luc-BPTF with indicated treatment in 293 T cells. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance