Fig. 7.

miR-26a-5p alleviate mechanic allodynia through a non-canonical Wnt signaling pathway. A–C mRNA levels of Ryk(A), CaMKII (B), NFAT(C) on day 3, 7 and 14 after surgery. Data are expressed as fold change compared to sham-operated controls. D–F Quantitative RT-PCR was used to assess the mRNA expression level of Ryk(D), CaMKII (E), NFAT(F). G Western blot was used to assess the level of Ryk, CaMKII, NFAT after Foxy-5 reverse and miR-26a-5p rescue. Representative immunoblots and quantification showing Foxy-5 reversed the effect of miR-26a-5p, while intrathecal injection of miR-26a-5p one day after Foxy-5 again rescued the effect of Foxy5. Data are expressed as fold change compared to the sham group. H–J Protein levels of IL-1β (H), IL-6 (I), and TNF-α (J), in the L4–L5 dorsal spinal cord of mice were tested by ELISA. n = 6 mice for each group. Data are represented as mean ± sem. *p < 0.05, **p < 0.01