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. 2022 Sep 7;41:267. doi: 10.1186/s13046-022-02482-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Circ_0000098 is highly expressed in HCC. A Venn diagrams from analysis of GSE155949 and GSE101850 datasets. B Illustration of circ_0000098 formation from SLC30A7 gene. C The stability of circ_0000098 and SLC30A7 mRNA was evaluated following RNase R treatment using RT-qPCR assays. D Assay of PCR-amplified products of circ_0000098 on an agarose gel electrophoresis. Divergent primers were capable of amplifying circ_0000098 inside cDNA but not genomic DNA (gDNA). GAPDH served as a linear reference, while convergent primers amplified both circ_0000098 and linear gDNA. E The back-splicing junction of circ_0000098 was validated using Sanger sequencing. F Comparison of circ_0000098 levels in HCC and adjacent normal tissues. G ROC curve analysis of circ_0000098 for distinguishing HCC from healthy tissues. H Kaplan–Meier curve analysis of the prognosis of HCC patients with high or low circ_0000098 expression. I RT-qPCR was used to detect circ_0000098 expression in HCC cells (HCC-LM3, BEL-7404, and SK-Hep1) and a normal cell line LO2. NS: not significant. ***P < 0.001