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YopN and YopD bind independently to Gst-TyeA. The experimental protocol described in Fig. 6 was used with Y. enterocolitica VTL1 (YopN−) carrying pLC199 and Y. enterocolitica VTL2 (YopD−) carrying pLC199. YopN copurified with Gst-TyeA in the absence of YopD, and YopD copurified with Gst-TyeA in the absence of YopN. See the legend to Fig. 6 for details.
