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. Author manuscript; available in PMC: 2023 Jul 21.
Published in final edited form as: Cell Chem Biol. 2022 Jun 15;29(7):1162–1173.e5. doi: 10.1016/j.chembiol.2022.05.008

Figure 5. HDAC7-dependent gene expression by AR using RT-PCR.

Figure 5.

(A) Wild type AR was co-expressed with wild type (WT) or GOF mutant (GF) HDAC7. Cells were then untreated (0.01% DMSO only) or treated with DHT (1 nM in 0.01% DMSO) for 36 hours. After lysis, total cDNA was synthesized from mRNA with reverse transcriptase and mRNA levels of SPRF5 and Wnt16 were measure by RT-PCR reaction from the total cDNA. Representative gel images show triplicate reactions from three independent trials. (B and C) Signals were quantified from part A using ImageJ 1.47v and normalized to the DHT alone sample (lane 2, set to 100%) to calculate mean percentage and standard error. Mean light intensity data and standard error from at least three independent trials are shown, with the data provided in Figure S8. Student t-test analysis was applied, where NS = not significant (p > 0.05), * = p < 0.05, ** = p < 0.01.